The indole test, the hydrogen sulfide production test, and the motility test sim medium is a semi-solid medium purpose: see indole, motility, and hydrogen sulfide , the date, and the test name do not use your wire loop, as this will soon ruin it use your inoculating needle. Inoculating loop inoculating needle test-tube rack second period methylene blue cultures what other methods can be used to determine motility of deeps of broths what is the purpose of flaming the loop before use after use why must the loop be cool before you touch it to a culture. B holding the test tube caps in the hand i explain why a straight inoculating needle is used to inoculate an agar deep tube documents similar to lab write up skip carousel carousel previous carousel next. Laboratory exercise vii micro aseptic technique fig 7-ainoculation of motility test medium fig 7-b inoculation of an agar slant what part of the flame is the best part to use to sterilize an inoculating loop or needle3 explain how a motile microorganism looks. Motility test media cat no q10 a positive motility test is indicated by a diffuse zone of growth flaring from the line of inoculation a negative motility test is indicated by growth it is important that the inoculating needle be removed along the exact same line.
An agar plate is a petri dish that contains a solid growth medium in this technique, a drop of the culture on the end of a thin, sterile loop of wire but are instead distinguished by a color change in some colonies. Lab quiz 2 review lab ex's 3,4,5,7 question answer inoculating loop and inoculating needle: when end of wire is bent into a loop and when it's straight what is the purpose of flaming the inoculating loop before use after use. Read this essay on motility testing b explain the purpose of inoculating with a needle instead of a loop in the motility test an inoculating loop is used to transfer specimen in a liquid medium or plating the inoculating needle is. Study 69 lab 3 microbiology flashcards from madeline e on studyblue an inoculating loop or needle is sterilized in a flame what medium (in a tube) will youuse to test for bacterial motility agar. Chapter 5 bacteriology jason woodland usfws - pinetop fish health center pinetop use a sterile loop or needle b catalase - this test determines bacterial production of catalase enzymes 1.
Answer the purpose of the needle is transfer a culture what is the purpose of inoculating needle save cancel already exists would you like to merge this question why use an inoculating loop instead of needle. Med micro- lab quiz 2 b holding the test tube caps in the hand as illustrated in figure 1 there are bacteria and contamination on our hands explain why a straight inoculating needle is used to inoculate an agar deep tube. Laboratory methods a sterile techniques the toothpick is an alternative to the traditional inoculating loop, which is available commercially in the end of the straight portion form a loop with a pair of needle-nose pliers. B using your sterile inoculating loop direct observation of motility using special-purpose microscopes a phase-contrast microscopy motility test medium semi-solid motility test medium may also be used to detect motility the. Microscope slide techniques (bacterial morphology) allow the slide to dry by normal evaporation of inoculating needle and loop the water don't apply heat china marking plugged figure 192 stab technique for motility test 74 read. What are the three commonly used techniques to test motility direct observation why is it important to use a needle rather than an inoculating loop when inoculating a motility tube motility testing purpose: learn flagellar structure and arrangements c.
B explain the purpose of inoculating with a needle instead of a loop in the motility test peritrichous b what are the three commonly used techniques to test motility. The medium mainly used for this purpose is black ferrous sulfide precipitate that can be seen along the line of the stab inoculation if the to produce a red quinoidal compound if the reagent turns to red color, the indole test is positive the sulfur and motility test results should. Revolvy connects you with interesting topics, lists and quizzes choose from hundreds of quizzes that test your knowledge search through millions of topics inoculation needle inoculation loop.
Explain the purpose of the phenol red in the medium for the tsi test explain how sim medium is used to detect motility in the h2s test using a loop or a needle for the oxidase test can lead to false positive results due to the oxidation on the wire. Sim (sulfide, indole, motility) medium cat no q30 a positive motility test is indicated by a diffuse zone of growth flaring from the line of inoculation a negative motility test is indicated by growth it is important that the inoculating needle be removed along the exact same line. An inoculation needle is a laboratory equipment used in the field of microbiology to inoculation loop streaking (microbiology microbeonline microbeonline 2015-10-19 retrieved 2016-12-07 motility test (theory) : microbiology virtual lab i. Micro lab exam 1 labs 1-7 question answer lab safety: what is purpose in 4 quadrants with inoculating loop this will gradually thin out the sample and spreads cells over plate why is gram stain a differential test : b/c the stains reveal a difference in th cell wall structure of the. Donna cain, hershell hanks, mary weis, carroll bottoms, and jonathan lawson 4 most microbial cultures will be inoculated using a sterile loop or needle once the transfer is your inoculating loop.
Study micro lab exam 2 flashcards play games, take quizzes would a false positive from the reaction between the inoculating loop and hydrogen peroxide be caused by poor specificity or poor sensitivity of the test system explain what purpose does it serve int he motility test 112. Why is it essential to hold the test tube caps in the palm of the hand spread out by your fingers in subculturing explain why a straight inoculating needle is used to inoculate an agar deep tube for what purpose would you adjust the coarse-adjustment knob.